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“you couldn’t get a good result this year..” ..she said .

“I know…but please listen to me….”

She : ” What else should i expect from you? You couldn’t get a good result.. couldn’t get into medicals… Do engineering at least…”

“But I don’t like maths… Or physics…its tough for me… ”

“Then what else would you do with your life? Everyone is going to say .. that my boyfriend is good for nothing ” she said .

“I did bad for just one year… please listen to me…”

“Please ….im done….. LEAVE ME ALONE I don’t want my destroy my future..” she said.

He felt a great shock… As the words “leave me alone” hit him like a thunderbolt.. straight through his heart.

Yes , he felt weak.. he couldn’t take it.He cried that day.. hid himself from everyone.. deleted all of his social media accounts. He wanted to be alone!…

So this all started back in 2015.

It was a bright morning … Wednesday , to be exact. He had his chemistry tution . But also he was excited and scared for something else. Later that day he returned from his tution and opened his Facebook , and treated himself with a surprise text message he received 1 hour ago. The text said .. ” yes i love you too.” He felt partly nice and scared again , as because of his conservative parents.. and also as he was a the most average looking boy around , which did lead to a certain amount of insecurity .But above that , it was time for celebration. As he got his only crush as his girlfriend.They met almost everyday , at tutions and at different locations.

Later that year he had his final exam of class 11..It went well.

Results came.. wasn’t the “best”…but no less than “better” .That afternoon… After meeting, she said ,” you got excellent marks in maths.. im sure you’ll go for engineering.. right?

He said : Umm…. No.. i would choose biology over every other subjects to study with.

She said : why? You got more in maths than in biology!!

He : I don’t love maths.. that marks is a result of regular practice throughout the year …but biology is what i love .!

She : i donno what you are upto.. but im happy for your marks though!!

He : and im happy because you are happy!!

And they laughed , making promises to stay together for ever.. little did they know about their future.

All these promises and love vanished after class12 th results.

He failed to meet her expectations. He was found guilty , as he wasted more time in love , than in studies to secure his own future.Neither he made it to any medical college.. nor did he wanted to do engineering.

They broke up!!

He made up his mind , to meet her after he achieved something in life!

After that day several years went by , he became busy in his studies.He continued his studies in zoology , completed BSc , MSc , and got a PhD .But there wasn’t a single day when he didn’t think about her.

Most people move on after breakup , but for him love wasn’t something to let go of , love is like a flower , It needs to to be taken care of and nurtured to make it beautiful and long lasting!

Every moment he thought .. “what would it be like when i meet her and say.. i have achieved my goals now nothing can separate us.. i established myself”

But life had other plans for him.

He went to a conference in kerala . The conference was on sunday , but he was there 4 days before to explore the place. In the afternoon while he was walking down the streets , he saw something that made his jaw drop. He saw his long lost love again. He wanted to make up for all the failures that he had brought to her . He saw her walking down from other side of the street. He wanted to surprise her.. so he quickly crossed the street , and followed her. She entered a road side shop to buy earrings. He sneaked behind her quietly and stood looking at her through the mirror in front .

She finally saw him from the mirror and looked back… She was shocked too. But a sign of anger was present on her face.

He said : ” you look beautiful in those earrings ”

“You??! Are you totally shameless? How did you even afford to come here? I hope you couldn’t do anything with your life!!” She said .

The boy wanted say a lot of things to her … But he wasn’t allowed to. She said again ” what are you now ? A taxi driver or what? Please just go away , and for your kind information , im married now.. my husband has a government job.. so please!! Leave!! … and never try to contact me again” .

The boy was stunned. All he hoped for was a second chance to prove himself in front of her!

Today he was truly hurt … Tears rolled down his eyes , he turned around and walked off without revealing his qualifications and achievements !!
His name ” Dr Arun Das ” was enough as a renowned geneticist. But he chose to remain silent.

Well he did get a second chance to continue his studies with zoology , but never really got a chance to prove her..that he did really loved and cared for her! He now has a well established life but failed to het back the love of his life.

I met this guy when i was in second year. He just got admitted to college.. the smell of freshmen is all around.

Our interactions with our juniors weren’t that much. But we had an upcoming excursion … a college trip , where less work and more fun occurs . I was actually excited for it , though it was our second trip from college.

But this time , both first year and second year students were about to attend it.

Sundarban it was. It is really an awesome place to visit , though we didn’t see any tigers .

So it was evening of the first day at sundarban , there was a knock at the door.

Me and my 4 friends were alloted in one room. The door was unlocked .. so i said .. “yea come in” .

The door opened .. and there stood a boy of about 5’9″ , slim , wearing shorts.. and a tee-shirt .. “asbo dada? ” , He said..

“Ha aye aye..bos “.. i said..

I wasn’t really prepared for an interaction with juniors.. neither any of my friends were.

Well this guy was a jolly one.. ” ki korcho tomra? Sokal sokal ready? Kokohn berote hobe?” He said and sat on the bed.

I said ” we will be going in few minutes.. ja ready ho.. ”

One of my friends said ” ki college kerom lagche? Zoology bhalo lage?”

The boy smiled and replied , ” ha ..college bhaloi lagche..eyi to sobe dhuklam college ”

After a small chitchat, he went off to get ready and we got busy in our work.

In any other case , i feel awkward to talk to unknown people, but this guy was a jolly one.. like.. we didn’t feel he was new to us.

That evening.. we were playing badminton…near our room.

I am always kind of lazy guy, i kept sitting at a place and watched everyone have fun.

At that time i saw that boy climb a small one storey building, it was then i was shocked.. i shouted ” ki korchis?? Pore jabi to !!”

The boy laughed.. ” na…gram erom praye gache uthte pari, khub moja hoy”

I remained silent for a moment , it was really hard for to me register the fact a boy is scaling a building , and at the same time i was scared that he might fall and hurt himself. Later i was proved wrong.. he wasn’t that weak either.

Well time flies,

After our excursion, our exams were near.. a few months were left for our exams , and may be first year finals were knocking at the door.

In the mean time.. i heard from one of my friends, ” bhai sunli ? Oi cheletar recently cancer dhora poreche”

I was startled.. i said ” what? Kon chele? Kon year? ”

“Oito gram er chele ta re “.. he said

I got shocked.. i couldn’t digest the fact..someone around me is having cancer.

I personally belong to a small town and there people are healthy.. and that boy .. was from a village..where people are also supposed to be healthy as it is free from pollution….. or may be at least less polluted.

I did text him on WhatsApp .. he said ” blood cancer hoeche”

And i heard he had financial problems .. as he wasn’t from a well to do family. Our seniors , juniors , proffesors and my batch mates together did raise a good sum of money..but nothing seemed to be enough for his treatment . He got admitted in a hospital in mumbai, far from his home.

A boy , as jolly as him , was being crushed under the weight of cancer treatment .

I saw his statuses on WhatsApp everyday.. on how much he was missing his home , his friends , his family.

He had a lot of problems among which , lack of blood supply was a mojor one.

But inspite of all these adversities.. i recently heard from him ” ayi mas e chuti hobe dada”

Remember what i said earlier…about me being scared whether he would hurt himself by falling off a small building because he was very skinny??(hypothetically)

Well he survived this harsh treatment to fight cancer..

You know why? Because he has a strong will…yes..he wasn’t weak at all.

Yes he defeated a war single handedly which continued for almost about 1 year. He now has a big smile on his face.

And me? I do really want to welcome him home when he will be returning..but now again my exams are about to start.

And i like the fact… That how fast , a total stranger village boy accelerated to be our most dearest and adorable junior.

Sometimes i do think.. there are researches going on everyday to cure cancer . Someday earth might be cancer free. But till then everybody has to be a brave fighter, because its not just a battle … Its a war.

“I don’t think the fabric is upto the mark.Better send these back and place order for new ones.”i said looking at the suits.

“Sir you do really care alot, are you sure you wanna sell this off? The new owner might not treat it as you do .”

I didn’t expect this..but i was flattered.

In the mean time i heard someone calling, “excuse me, can you show some wedding suits ??”

I turned around just to surprise me..i saw her..

her left eye brow rose.. and she again said

” you? Well good enough.. its my wedding this year.”

“Oh! Great !” I said.

“Now come on show me some expensive suits for my would be husband..” said she.

I looked at the boy … For a second.. and then turned to reach out for the suits..

then.., “sir you don’t have to do it, we are here we will show them”

I smiled..” its ok..let me do this once.”

I took out the suits and showed her…

She did like them..

I could read it from her face she was about to bargain for price.. but i added ” its on 75% discount MAM… ”

“Oh! Well then…i would like to have this two..please do the billing” she said.

I sent over the suits for packing and billing..and while she was at the billing counter..i left the shop and the mall it was in.

I sent off my driver , drove back home myself…. And lay down on the bed.

“She is getting married! Well the would be husband didn’t look that bad…. I hope he is good….and im sure he is in every aspect better than me.” I thought..

Well i did give her a wedding gift.. two suits worth 1lakh rupees which weren’t on sale. I won’t say i am not happy…but i am shocked.

Its been so long since we broke up… Since i last saw her.Never got a chance to prove myself worthy for her anymore..got busy to prove her wrong… But didn’t actually get a chance to prove.

My phone rang …. “Sir your meeting starts in 5 minutes” ..

“Ok! Ill be there!

I washed my face..got dressed up..

Well it was a meeting with the guy i wanted to sell the mall to.

My manager and me..both got into the car..

Manager asked ” sir he is willing to pay a price more than we talked about”

I remained silent. Actually i couldn’t come out of the shock of “im getting married” said by her this morning.

We reached our location .

At the meeting, everything was going nice.

I still couldn’t make my mind whether to sell it or not..

Then the final papers were put forward for me to sign on it.

I sat silently.

Manager said ” sir ! The papers…”

I stood up… ” Gentleman… The deal is cancelled..i am sorry for bothering you”

The room got filled with chaos as i left through the door.

Later at home… At dinner table… My mom again for the nth time.. said ” don’t you think you should get married now?? All of your friends are married… ”

I said..” yea.. i think now i should”

I should move on… It really did hurt today.

No i didn’t sell the mall .. now that i have a good memory to cherish…how could i? I saw her after about 20 years later….gave her a wedding gift…

i could have sold any other malls i possess but not that. May be she thought i was a just a mere salesman..but its kinda ok.

I guess its time to move on.

“what are you going to wear tommorow?” She asked.

Well he did expect that… ” Blue jeans and a black T-shirt… I guess ” he replied..

She : “why? Tommorow is Saraswati Puja.. you should wear punjabi.. it would be great”

He wasn’t a big fan of indian traditional dresses.. he never wore a “punjabi before”

“Why? What’s bad in jeans and tshirt?” He said..

“Ok do whatever you feel like i won’t interfere” she said..and went offline.

He did sense the anger presented through the text. Giving the whole dressing stuff a second thought he decided to wear punjabi.

Next day morning, it was Saraswati puja. Every year people wait for this day with a lot of plans . Some people on this day pray to god for a better future and some spend the day with their special ones and friends.

The boy wore punjabi for the first time in life. He was uncomfortable in it..but still wanted to see her reaction.

He wen to the destination and waited.. after a long wait, which apparently seemed to ve longer than he thought, he got a glimpse of her.. draped in blue saree, hair curled up and left open.. a red bindi on her forehead.. and the most beautiful ornament of all.. a sweet smile on her face.

He was mesmerized . She walked up to him , said ” ki dekcho?”

He said , ” na kichu na, lets go inside”

They both went inside.. it was their first Saraswati puja together.

They stood , together for as the puja continued both stealing their eyes on each other.

The boy today didn’t ask for a better number for upcoming exams.. neither he asked for a better carrier or for extra knowledge. He didn’t actually ask for anything. He just said ” thank you , for such a nice gift this morning “.

The girl turned towards him and asked , ” kemon lagche amae?” The boy didn’t really answer ; looked at her blankly. She asked again, “ki go? How am i looking?”

Her sweet voice slowly turned into the unbearable noise of alarm.

He woke up , checking the watch , it was 9:40 am.

He got up from his chair , closed his books, walked towards the wondow.

A slight cold Breeze touched his face . H looked outside, saw new couples walking by .. dressed in indian outfit.

Why not? After all it was Saraswati Puja .. and it is the Bengali Valentine’s day.

Knowing his mother would give him a call for the puja he went ahead to start his day.

Sometimes i really go speechless by seeing things happening around me.

Life has a value..that nobody can pay for. Its the most precious thing god has ever gifted to earth.

Every living organism has an equal right to continue its life on this planet . We are nobody to put any boundary or limit to it.

Some people might think. “Oh look at that puppy, lets pelt some stones at it” .. others may think , ” lets torture the kitten “. No! that’s not funny, playing with lives isn’t funny.

In 5 years about 19028 cases of animal cruelty are found in some states of india!

Animals aren’t doing any harm to us.. then why should we torture them?

And yet again in 2019 it wasn’t such a happy start for animals . 2 nursing students brutally killed 16 puppies at a hospital in kolkata . Why did they even do so? Didn’t their hands shake at all? They simply took away lives of innocent puppies that are new in this world .

It takes 58-68 days for a dog to give birth. It takes so long to create life .. in some animals its even more. But some nursing students ended it in few minutes.

Dogs are regarded as man’s best friend. Well i don’t think this is the way to treat best friends

Not just dogs, even cats and other animals are tortured. Take for example poachers brutally kill wild animals to to sell their body parts.

Think about it how would you feel like if you were in their place????well it won’t be a pleasant experience…

I would really like to clearly send this message to everyone out there.. its high time to stand against animal abuse.

Help by signing this petition…to punish those who took away 16 innocent lives..

http://chng.it/TkRPyjv2

I personally believe that… :

Its our work that defines us as a human.

Everyone is kinda unique , genetically , physically , mentally.. .

So getting back to the topic…. about which i wanted to write… Is about skin colour.

An individual’s skin colour is the result of genetics . Well this constitutes the individuals genetic makeup and exposure of skin to sun.

Now the main part .. the skin colour is regulated by many factors. But the most important being MELANIN .

Melanin

Produced from : Melanocytes

Colour : its presence is responsible for darker skin colour

Process of formation : melanogenesis. Melanin is produced by oxidation of amino acid tyrosin followed by polymerization

Enzyme required for production : Tyrosinase

Types : • Eumelanin -brown colour

• Pheomelanin – pink to red hue

Fig : Melanocyte
Well the number of melanocytes are present in almost same numbers in both dark skinned and fair skinned people . But the skin colour differs in the amount of melanin produced by those cells .

Well …. There are perks of having melanin in your skin. Melanin protects the skin form UV rays by absorption and reduces the risk of incurring DNA damage leading to cancer and also help to retain folate reserves of body.

People towards the equator tend to have more darker skin colours as there is a high exposure to sun rays.

This may be result of long term adaptation!

Yep…. i guess in a way this makes dark skin to be more advantageous than fair skin. Or may be having dark skin i.e high amount of melanin , may also be a way of protection appointed by our body to remain protected against UV rays.

To sum it up .. lets leave the rest of the evolutionary process to time itself and wait and watch what the future holds .

If something comes to life in others because of you, then you have made an approach to immortality

–Norman Cousins

Ya being immortal sounds good.. but there is a bit of a darker side to the whole “immortality is awesome” thing.

Lets just say a human on earth has become immortal, meaning he is having an awesome time living life… for lets just say millions of years. Like gathering memories and knowledge for several years .That does sound good , but let’s get to the real fact… Human brains have a capacity of 2.5petabytes that is about million gigabyte. Eventually this space would run out too(about 300years).. it might sound awkward but it would run out when you are constantly storing memories of everyday every moment.This would lead to a point when you won’t be able to take in anything else. Due to this the man living for millions of years would not be able to even speak a complete sentence properly due this problem.

Ya that’s sad and not good too for someone being the oldest guy to live on earth.

Another fact human beings are very good at manipulating their resources required to survive .

Initially a normal human population graph may start exponentially but later it might change to logistic growth due to resource manipulation.

The plateau formed in the logistic growth curve denotes a population size maintained almost at a constant state .. that is number of deaths and number of births are more or less balanced.

Now, lets just say humans have attained immortality, the exponential graph would never change to logistic graph . It would remain exponential and exceed carrying capacity . Thus it would result in fight for resources . Population will increase to an extent that earth would not be enough for them.

For a good ending , it would not be a problem if humans constantly keep colonizing one planet or the other . For now let’s just see what the future holds.

May be you have a practical exam or you just want to do it out of curiosity.

This method is haematoxylin-eosin staining method.

Here is a easy way to do so..

1. Take the slide with unstained tissue.
2. Dip it in xylol , keep for 5 min. (To remove paraffin)
3. Now take it out and dip it into absolute alcohol, keep for 5 minute.
4. Then transfer it to 90% alcohol, keep for 5min.
5. Then keep transferring to 70% to 50% to 30% . Each should be kept for 5 minute.
6. Now transfer it to distilled water and keep for 5 minute.
7. Then transfer it to haematoxylin stain. The time required depends on how strong the stain is, it might be as fast as 2-10 seconds or as slow as 5-10 minute.
8. Then , if properly stained (observe violet color against a white background) , transfer it to coupling jar with tap water.
9. Now it is transferred to 30% alcohol then to 50% to 70% to 90% alcohol. Keep for 10 minute in each.
10. From 90% alcohol , transfer it to eosin stain. Again the time required to keep in stain may vary depending on how strong it is.. may take a few seconds to few minutes.
11. After eosin , dip it in another 90% alcohol and immediately transfer to Absolute alcohol and keep for 10-15 minute.
12. Then transfer to xylol again (as a precursor of dpx).
13. On a cover slip take a drop of dpx and mount it on slide(after taking out slide from Xylol)
14. Let it dry.

Section of thyroid

On moving from 90% to 30% grades of alcohol we are hydrating the tissue in slide.

On moving from 30% to 90% grades of alcohol we are dehydrating the tissue in slide.

Here’s a video of tissue staining:https://youtu.be/nG1MnkIQf_M